Efficient In Vitro Cultivation of Mouse Cochlear Hair Cells

September 15th 2023


* Diese Autoren haben gleichermaßen beigetragen


Summary

Herein, we present a detailed protocol for isolating and culturing primary cochlear hair cells from mice. Initially, the organ of Corti was dissected from neonatal (aged 3-5 days) murine cochleae under a microscope. Subsequently, cells were enzymatically digested into a single-cell suspension and identified using immunofluorescence after several days in culture.

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