Anmelden

Macau University of Science and Technology

2 ARTICLES PUBLISHED IN JoVE

Biology

A Non-Degradative Extraction Method for Molecular Structure Characterization of Bacterial Glycogen Particles

Jun-Jiao Wang^*1, Meng-Meng Wang^*2,3, You-Wei He⁴, Zhang-Wen Ma^2,3, Zi-Yi Wang^5,6,7, Rui Qiao^2,3, Xin-Le Tan^5,6, Qing-Hua Liu⁸, Liang Wang^1,2

¹Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, ²Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, ³Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, ⁴Department of Biological Sciences, School of Life Sciences, Xuzhou Medical University, ⁵School of Chemistry and Molecular Biosciences, The University of Queensland, ⁶Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, ⁷Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, ⁸State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology

Bacterial glycogen structure is greatly impacted by extraction methods which may result in molecular degradation and/or biased sampling. It is essential to develop methods to minimize these problems. Here, four extraction methods have been compared using size distribution and chain length distribution as key criteria for minimizing extraction artifacts.

Cancer Research

Surgical Transplantation of Tumor Cells into the Spinal Cord of Mice

Xun Mingjuan^*1,2,3, Lin Han^*4,5, Yu Caiyuan^1,6,7, Pang Bo⁴, Wang Yongzhi^4,5, Yan Jun^1,2,3

¹Beijing Institute of Brain Disorders, Capital Medical University, ²Laboratory of Brain Disorders, Ministry of Science and Technology, Capital Medical University, ³Collaborative Innovation Center for Brain Disorders, Capital Medical University, ⁴Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, ⁵Department of Molecular Neuropathology, Beijing Neurosurgical Institute, Capital Medical University, ⁶State Key Laboratory of Quality Research in Chinese Medicines, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, ⁷Faculty of Agroforestry and Medicine, The Open University of China

The present protocol describes the development of a reproducible murine model of spinal cord glioma by injecting tumor cells into the intervertebral space, offering a more effective and less invasive approach for research and therapeutic development.