Anmelden

Center for Infectious Disease Research

2 ARTICLES PUBLISHED IN JoVE

Biology

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Description of a quantitative phosphorylation procedure using cryolysis, urea solubilziation, HILIC fractionation and IMAC enrichment of phosphorylated peptides.

Biology

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

Thurston Herricks¹, Fred D. Mast^1,2, Song Li¹, John D. Aitchison^1,2

¹Institute for Systems Biology, ²Center for Infectious Disease Research

We present a method for quantifying growth phenotypes of individual yeast cells as they grow into colonies on solid media using time-lapse microscopy termed, One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). Population heterogeneity of genetically identical cells growing into colonies can be directly observed and quantified.