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Institute for Pathology "Victor Babes"

2 ARTICLES PUBLISHED IN JoVE

Medicine

Minimal Invasive Surgical Procedure of Inducing Myocardial Infarction in Mice

Adelina Curaj¹, Sakine Simsekyilmaz¹, Mareike Staudt¹, Elisa Liehn¹

¹Institute for Molecular Cardiovascular Research, RWTH Aachen University, Germany

A highly reproducible model for myocardial infarction in mice with minimal invasive manipulations is described. The model can be easily performed, resulting in a high reproducibility and survival rate. Thus, the described model will reduce the number of required animals as requested by the 3R principle (Replacement, Refinement and Reduction).

Medicine

Implantation of Human-Sized Coronary Stents into Rat Abdominal Aorta Using a Trans-Femoral Access

Anne Cornelissen^*1, Roberta Florescu^*1, Nicole Schaaps¹, Mamdouh Afify¹, Sakine Simsekyilmaz¹, Elisa Liehn^*1,2,3, Felix Vogt^*1

¹Division of Cardiology, Angiology, and Critical Care, University Hospital RWTH Aachen, ²Department for Operative Intensive Medicine, University Hospital RWTH Aachen, ³Institute for Pathology "Victor Babes"

This protocol describes the implantation of human coronary stents into the abdominal aorta of rats with an apoE^-/- background using a trans-femoral access. Compared with other animal models, murine models carry the advantages of high throughput, reproducibility, ease of handling and housing, and a broad availability of molecular markers.