JoVE Logo

Sign In

BloodCenter of Wisconsin

4 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Depletion of Specific Cell Populations by Complement Depletion
Bonnie N. Dittel 1
1BloodCenter of Wisconsin, Blood Research Institute

To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.

image

Immunology and Infection

Isolation of Mouse Peritoneal Cavity Cells
Avijit Ray 1, Bonnie N. Dittel 1
1BloodCenter of Wisconsin, Blood Research Institute

The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells. Here we provide a comprehensive method for the isolation of peritoneal cavity cells in the mouse.

image

Immunology and Infection

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
Sreemanti Basu 1, Hope M. Campbell 1, Bonnie N. Dittel 1, Avijit Ray 1
1Blood Research Institute, BloodCenter of Wisconsin

For many scientific studies requiring a biological and chemical analysis of cell populations the cells must be in a high state of purity. Fluorescence activated cell sorting (FACS) is a superior method in which to obtain pure cell populations.

image

Biology

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice
Magdalena Sobczak 1, Jillian Dargatz 1, Magdalena Chrzanowska-Wodnicka 1
1Blood Research Institute, BloodCenter of Wisconsin

Here, we describe a protocol for isolation and culture of murine pulmonary endothelial cells. This method comprises mechanic and enzymatic lung tissue dissociation as well as a 2-step purification process using anti-PECAM-1 and anti-ICAM-2 antibodies conjugated to magnetic beads, which produces a pure endothelial cell population of mostly microvascular origin.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved