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Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

DOI :

10.3791/2316-v

10:46 min

December 14th, 2010

December 14th, 2010

46,298 Views

1Blood Research Institute, BloodCenter of Wisconsin

Here, we describe a protocol for isolation and culture of murine pulmonary endothelial cells. This method comprises mechanic and enzymatic lung tissue dissociation as well as a 2-step purification process using anti-PECAM-1 and anti-ICAM-2 antibodies conjugated to magnetic beads, which produces a pure endothelial cell population of mostly microvascular origin.

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Isolation

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