preparation of mice and carrageenan solution for creating a model of inflammatory pain

<meta charset="utf8"></head><body>Doppelte Hinterpfoteninjektion für verbesserte analgetische Tests

Pain is a public health problem worldwide. It is triggered by noxious thermal, mechanical, or chemical stimulation. It is difficult to perform pain studies in humans because of ethical concerns. Therefore, using animal pain models is a key approach to understanding pain mechanisms and research treatments1. The hot plate test has been widely used for the assessment of thermal nociception and hyperalgesia2. Currently, the model of inflammatory pain used for the hot plate test is induced with an intraplantar injection of carrageenan in one hind paw. For example, to investigate the antinociceptive effect of rotigotine-loaded microspheres, an animal model of inflammatory pain was prepared by a single-hind-paw injection3. To assess the effects of catechol-o-methyl-transferase inhibitors on thermal nociception in mice, carrageenan was administered into the plantar region of the right hind paw4. To investigate the analgesic role of the extract of Posidonia oceanica (L.) Delile, an inflammatory pain model was prepared in mice by intraplantar injection of carrageenan in the right hind paw5. However, the findings from our laboratory showed that a mouse with the single-hind-paw injection of carrageenan would lift its paw to avoid thermal nociception, which inaccurately extends the animal's response to a thermal stimulus in the hot plate test. Thus, we hypothesized that the inflammatory pain model with the single-hind-paw injection of carrageenan could not accurately reflect the thermal pain threshold. Therefore, we optimized the method by injecting carrageenan into both hind paws to establish a modified inflammatory pain model. 
The mice subjected to both-hind-paw injection of carrageenan had no way to avoid the thermal stimulus for both hind paws at the same time. The pain threshold was defined as the time from placing the mouse on the hot plate to the mouse licking either hind paw. The results confirmed that the modified inflammatory pain model is more suitable for evaluating the nociceptive temperature threshold. This may improve the efficacy of the hot plate test for the research and development of analgesic drugs.

<meta charset="utf8"></head><body>Autor im Rampenlicht: Verbesserte Methode zur Bewertung analgetischer Wirkungen – Injektion von Carrageen mit doppelter Hinterpfote bei Mäusen

Ein modifiziertes Modell für entzündliche Schmerzen zur Untersuchung der analgetischen Wirkung bei Mäusen

Herstellung von Mäusen und Carrageen-Lösung zur Erstellung eines Modells für entzündliche Schmerzen

preparation-mice-carrageenan-solution-for-creating-model-inflammatory

Research

JoVE Journal

Neuroscience

Preparation of Mice and Carrageenan Solution for Creating a Model of Inflammatory Pain

186 Aufrufe. Beginnen Sie mit der Verwendung von weiblichen ICR-Mäusen mit einem Gewicht von 20 bis 25 Gramm für die Experimente. Lassen Sie die Tiere drei Tage lang an die Versuchsraumumgebung gewöhnen, bevor Sie mit den Versuchen beginnen. Um Carrageenlösung herzustellen, wiegen Sie 0,1 Gramm Carrageen, geben Sie 10 Milliliter sterilisierte normale Kochsalzlösung in ein Röhrchen, geben Sie 0,1 Gramm Carrageen in das Röhrchen und wirbeln Sie das Röhrchen 30 Sekun...

Serie: Herstellung von Mäusen und Carrageen-Lösung zur Erstellung eines Modells für entzündliche Schmerzen

A Modified Inflammatory Pain Model to Study the Analgesic Effect in Mice

The hot plate test is widely used to evaluate analgesic effects on inflammatory pain in mice. A commonly used model of inflammatory pain was induced with an intraplantar injection of carrageenan in one hind paw. However, the findings from our laboratory showed that mice with a single-hind-paw injection of carrageenan lifted their paws to avoid thermal nociception during the hot plate test. Because of this response,&#160;previous injection method cannot accurately reflect the thermal pain threshold. Thus, we investigated a new method to avoid this issue. In the present study, we modified the previous method by injecting carrageenan into both hind paws to establish the model of inflammatory pain. The results demonstrated that both-hind-paw injection with carrageenan was sensitive and a better method to induce inflammatory pain when using the hot plate test than single-hind-paw injection. On the basis of these findings, we designed further experiments in which mice with either both-hind-paw or single-hind-paw injection of carrageenan were treated intragastrically with celecoxib (30 mg/kg). The results of the hot plate test showed that celecoxib augmented the thermal pain threshold in mice with both-hind-paw injection of carrageenan but not in mice with single-hind-paw injection of carrageenan. In summary, we developed a superior method to induce a model of inflammatory pain to evaluate analgesic effect.

Here, we present a modified inflammatory pain model with the both-hind-paw carrageenan injection to evaluate the analgesic effect.

The hot plate test is widely used to evaluate analgesic effects on inflammatory pain in mice. A commonly used model of inflammatory pain was induced with ...