Biofilm Inoculum Infection, Bioluminescence Expression Assessment, and Wound Measurement and Imaging
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Results: Representative Bioluminescence Expression, Biofilm Evolution, and Wound Closure Analyses
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Conclusion
Transkript
The overall goal of this novel model is to allow real time in vivo monitoring of Biofilm wound infection and healing in diabetic mice. This method can help answer key questions in the wound healing field such as what are they underlying interactio
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Here we describe a novel diabetic murine model utilizing hairless mice for real-time, non-invasive, monitoring of biofilm wound infections of bioluminescent Pseudomonas aeruginosa. This method can be adapted to evaluate infection of other bacterial species and genetically modified microorganisms, including multi-species biofilms, and test the efficacy of antibiofilm strategies.