Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide

0:48

Lipid Extraction from Adipose Tissue

2:31

Preparation of Fatty Acid Methyl Esters (FAMEs) and GCMS Analysis

4:08

Deuterium Acetone Exchange of Plasma Samples to Determine Body Water Enrichment

5:04

Results: Plasma Body Water Enrichment from D₂O, and Brown Adipose Tissue Total Fatty Acid Enrichment and Synthesis after 3 Days of D₂O Administration

6:34

Conclusion