Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium OxideRory Turner *1, Rajib Mukherjee *2, Martina Wallace 1, Joan Sanchez-Gurmaches 2,3,4
1UCD Conway Institute and UCD Institute of Food and Health, School of Agriculture and Food Science, University College Dublin, 2Division of Endocrinology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 3Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 4Department of Pediatrics, University of Cincinnati College of Medicine
Here, we present an inexpensive quantitative method utilizing deuterium oxide and gas chromatography mass spectrometry (GCMS) for the analysis of total fatty acid de novo lipogenesis in brown adipose tissue in vivo.
