Alessandra Stefan

Water-in-oil macroemulsions sustain long-term viability of microbial cells in organic solvents.

Silencing of the gene coding for the epsilon subunit of DNA polymerase III slows down the growth rate of Escherichia coli populations.

Efficient silencing of the gene coding for the epsilon subunit of DNA polymerase III in Escherichia coli is triggered by antisense RNAs featuring stability in vivo.

Artificial antisense RNAs silence lacZ in E. coli by decreasing target mRNA concentration.

Proteolysis of the proofreading subunit controls the assembly of Escherichia coli DNA polymerase III catalytic core.

Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli.

The regulatory elements of araBAD operon, contrary to lac-based expression systems, afford hypersynthesis of murine, and human interferons in Escherichia coli.

Shine-Dalgarno sequence enhances the efficiency of lacZ repression by artificial anti-lac antisense RNAs in Escherichia coli.

pGOODs: new plasmids for the co-expression of proteins in Escherichia coli.

Overexpression and purification of the recombinant diphtheria toxin variant CRM197 in Escherichia coli.

Stabilization of the Escherichia coli DNA polymerase III ε subunit by the θ subunit favors in vivo assembly of the Pol III catalytic core.

Continuous enzyme-coupled assay of phosphate- or pyrophosphate-releasing enzymes.

A synthetic post-transcriptional controller to explore the modular design of gene circuits.