Bert Poolman

Physiological response of Lactobacillus plantarum to salt and nonelectrolyte stress.

Mechanism of osmotic activation of the quaternary ammonium compound transporter (QacT) of Lactobacillus plantarum.

Phosphorylation and functional properties of the IIA domain of the lactose transport protein of Streptococcus thermophilus.

Glycine betaine transport in Lactococcus lactis is osmotically regulated at the level of expression and translocation activity.

Specificity mutants of the binding protein of the oligopeptide transport system of Lactococcus lactis.

Kinetics and substrate specificity of membrane-reconstituted peptide transporter DtpT of Lactococcus lactis.

Osmoregulated ABC-transport system of Lactococcus lactis senses water stress via changes in the physical state of the membrane.

On the binding mechanism of the peptide receptor of the oligopeptide transport system of Lactococcus lactis.

Combinatorial peptide libraries reveal the ligand-binding mechanism of the oligopeptide receptor OppA of Lactococcus lactis.

The lactose transport protein is a cooperative dimer with two sugar translocation pathways.

On the osmotic signal and osmosensing mechanism of an ABC transport system for glycine betaine.

Mapping of the dimer interface of the Escherichia coli mannitol permease by cysteine cross-linking.

Oligomeric state of membrane transport proteins analyzed with blue native electrophoresis and analytical ultracentrifugation.

How do membrane proteins sense water stress?

Quaternary structure and function of transport proteins.

Spatial organization of bacteriorhodopsin in model membranes. Light-induced mobility changes.

Combined in-gel tryptic digestion and CNBr cleavage for the generation of peptide maps of an integral membrane protein with MALDI-TOF mass spectrometry.

Transporters and their roles in LAB cell physiology.

ABC transporters: one, two or four extracytoplasmic substrate-binding sites?

The ATP/substrate stoichiometry of the ATP-binding cassette (ABC) transporter OpuA.

Identification of the dimer interface of the lactose transport protein from Streptococcus thermophilus.

On the role of the two extracytoplasmic substrate-binding domains in the ABC transporter OpuA.

Evaluation of the flow-dialysis technique for analysis of protein-ligand interactions: an experimental and a monte carlo study.

The binding specificity of OppA determines the selectivity of the oligopeptide ATP-binding cassette transporter.

Lipid-mediated light activation of a mechanosensitive channel of large conductance.

Bacterial osmosensing: roles of membrane structure and electrostatics in lipid-protein and protein-protein interactions.

Two homologous oligopeptide binding protein genes (oppA) in Lactococcus lactis opp2 [corrected].

Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles.

Lactococcus lactis uses MscL as its principal mechanosensitive channel.

The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membrane.

Substrate specificity and ionic regulation of GlnPQ from Lactococcus lactis. An ATP-binding cassette transporter with four extracytoplasmic substrate-binding domains.

Stoichiometry and substrate affinity of the mannitol transporter, EnzymeIImtl, from Escherichia coli.

Functional interactions between the subunits of the lactose transporter from Streptococcus thermophilus.

Specificity and selectivity determinants of peptide transport in Lactococcus lactis and other microorganisms.

Substrate-induced conformational changes in the membrane-embedded IIC(mtl)-domain of the mannitol permease from Escherichia coli, EnzymeII(mtl), probed by tryptophan phosphorescence spectroscopy.

The activity of the lactose transporter from Streptococcus thermophilus is increased by phosphorylated IIA and the action of beta-galactosidase.

Engineering covalent oligomers of the mechanosensitive channel of large conductance from Escherichia coli with native conductance and gating characteristics.

ABC transporter architecture and regulatory roles of accessory domains.

Functional analysis of detergent-solubilized and membrane-reconstituted ATP-binding cassette transporters.

The riboflavin transporter RibU in Lactococcus lactis: molecular characterization of gene expression and the transport mechanism.

A sensor for intracellular ionic strength.

The oligomeric state and stability of the mannitol transporter, EnzymeII(mtl), from Escherichia coli: a fluorescence correlation spectroscopy study.

Ion specificity and ionic strength dependence of the osmoregulatory ABC transporter OpuA.

On the decrease in lateral mobility of phospholipids by sugars.

Dual-color fluorescence-burst analysis to probe protein efflux through the mechanosensitive channel MscL.

Biochemistry. A missing link in membrane protein evolution.

The lipid dependence of melittin action investigated by dual-color fluorescence burst analysis.

Protein mobility and diffusive barriers in Escherichia coli: consequences of osmotic stress.

High-throughput cloning and expression in recalcitrant bacteria.

Probing receptor-translocator interactions in the oligopeptide ABC transporter by fluorescence correlation spectroscopy.

Membrane reconstitution of ABC transporters and assays of translocator function.

Quality control of overexpressed membrane proteins.

9th International Symposium on Lactic Acid Bacteria.

Dual-color fluorescence-burst analysis to study pore formation and protein-protein interactions.

Transport and sorting of the solanum tuberosum sucrose transporter SUT1 is affected by posttranslational modification.

On the mechanism of pore formation by melittin.

Proteomic analysis of secreted membrane vesicles of archaeal Sulfolobus species reveals the presence of endosome sorting complex components.

The yeast vacuolar membrane proteome.

Domain complementation studies reveal residues critical for the activity of the mannitol permease from Escherichia coli.

Matching the proteome to the genome: the microbody of penicillin-producing Penicillium chrysogenum cells.

The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter.

Nuclear transport factor directs localization of protein synthesis during mitosis.

The structural basis for peptide selection by the transport receptor OppA.

Engineering of ion sensing by the cystathionine beta-synthase module of the ABC transporter OpuA.

A method for site-specific labeling of multiple protein thiols.

Selenomethionine incorporation in proteins expressed in Lactococcus lactis.

The role of biomacromolecular crowding, ionic strength, and physicochemical gradients in the complexities of life's emergence.

Lateral diffusion of membrane proteins.

Proteomics of Saccharomyces cerevisiae Organelles.

Production of membrane proteins in Escherichia coli and Lactococcus lactis.

A karyopherin acts in localized protein synthesis.

A structural classification of substrate-binding proteins.

Purification and functional reconstitution of the bacterial protein translocation pore, the SecYEG complex.

Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis.

Ligand binding and crystal structures of the substrate-binding domain of the ABC transporter OpuA.

Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins.

Genome sequences of Lactococcus lactis MG1363 (revised) and NZ9000 and comparative physiological studies.

Influence of hydrophobic mismatch and amino acid composition on the lateral diffusion of transmembrane peptides.

Lateral diffusion of membrane proteins: consequences of hydrophobic mismatch and lipid composition.

Apex peptide elution chain selection: a new strategy for selecting precursors in 2D-LC-MALDI-TOF/TOF experiments on complex biological samples.

Macromolecule diffusion and confinement in prokaryotic cells.

Lipid packing drives the segregation of transmembrane helices into disordered lipid domains in model membranes.

Quaternary structure of SecA in solution and bound to SecYEG probed at the single molecule level.

De novo design of supercharged, unfolded protein polymers, and their assembly into supramolecular aggregates.

The molecular basis for antimicrobial activity of pore-forming cyclic peptides.

Structural basis for the enhanced activity of cyclic antimicrobial peptides: the case of BPC194.

Long unfolded linkers facilitate membrane protein import through the nuclear pore complex.

Importance of a hydrophobic pocket for peptide binding in lactococcal OppA.

Design and synthesis of ATP-based nucleotide analogues and profiling of nucleotide-binding proteins.

The structural basis of modularity in ECF-type ABC transporters.

Efficient overproduction of membrane proteins in Lactococcus lactis requires the cell envelope stress sensor/regulator couple CesSR.

Cystathionine β-synthase (CBS) domains 1 and 2 fulfill different roles in ionic strength sensing of the ATP-binding cassette (ABC) transporter OpuA.

The response of Lactococcus lactis to membrane protein production.

Evaluation of pulsed-FRAP and conventional-FRAP for determination of protein mobility in prokaryotic cells.

The transport of integral membrane proteins across the nuclear pore complex.

Functional characterization of amphipathic α-helix in the osmoregulatory ABC transporter OpuA.

Effect of iTRAQ labeling on the relative abundance of peptide fragment ions produced by MALDI-MS/MS.

Structural divergence of paralogous S components from ECF-type ABC transporters.

Structure and mode of peptide binding of pheromone receptor PrgZ.

Impact of Lactobacillus plantarum sortase on target protein sorting, gastrointestinal persistence, and host immune response modulation.

On the role of individual subunits in MscL gating: "all for one, one for all?".

Quantitative analysis of membrane protein transport across the nuclear pore complex.

Dual action of BPC194: a membrane active peptide killing bacterial cells.

Physicochemical factors controlling the activity and energy coupling of an ionic strength-gated ATP-binding cassette (ABC) transporter.

Functional diversity of tandem substrate-binding domains in ABC transporters from pathogenic bacteria.

Evolved Escherichia coli strains for amplified, functional expression of membrane proteins.

Lactococcus lactis YfiA is necessary and sufficient for ribosome dimerization.

Regulation of acetate kinase isozymes and its importance for mixed-acid fermentation in Lactococcus lactis.

Bacillus subtilis spore protein SpoVAC functions as a mechanosensitive channel.

Susceptibility to COPD: differential proteomic profiling after acute smoking.

Systematic identification of tRNAome and its dynamics in Lactococcus lactis.

Impact of osmotic stress on protein diffusion in Lactococcus lactis.

Disaccharides impact the lateral organization of lipid membranes.

Intrinsically disordered linker and plasma membrane-binding motif sort Ist2 and Ssy1 to junctions.

Conformational dynamics in substrate-binding domains influences transport in the ABC importer GlnPQ.

Size-dependent leak of soluble and membrane proteins through the yeast nuclear pore complex.

A sensor for quantification of macromolecular crowding in living cells.

Single liposome analysis of peptide translocation by the ABC transporter TAPL.

In memoriam: Wilhelmus Nicolaas Konings (1937-2014).

Protein costs do not explain evolution of metabolic strategies and regulation of ribosomal content: does protein investment explain an anaerobic bacterial Crabtree effect?

Engineering Escherichia coli for functional expression of membrane proteins.

Emergence of life: Physical chemistry changes the paradigm.

Active Nuclear Import of Membrane Proteins Revisited.

Watching conformational dynamics of ABC transporters with single-molecule tools.

Relative Rates of Amino Acid Import via the ABC Transporter GlnPQ Determine the Growth Performance of Lactococcus lactis.

A Plasma Membrane Association Module in Yeast Amino Acid Transporters.

Asymmetry in inward- and outward-affinity constant of transport explain unidirectional lysine flux in Saccharomyces cerevisiae.

On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli.

An updated structural classification of substrate-binding proteins.

On the Spatial Organization of mRNA, Plasmids, and Ribosomes in a Bacterial Host Overexpressing Membrane Proteins.

Microorganisms maintain crowding homeostasis.

Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding.

Ionic Strength Sensing in Living Cells.

A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy.

Proton-solute coupling mechanism of the maltose transporter from Saccharomyces cerevisiae.

Ribosome surface properties may impose limits on the nature of the cytoplasmic proteome.

Combined engineering of disaccharide transport and phosphorolysis for enhanced ATP yield from sucrose fermentation in Saccharomyces cerevisiae.

Real-Time Conformational Changes and Controlled Orientation of Native Proteins Inside a Protein Nanoreactor.

Steric exclusion and protein conformation determine the localization of plasma membrane transporters.

Energy Coupling Efficiency in the Type I ABC Transporter GlnPQ.

Protein Linkers Provide Limits on the Domain Interactions in the ABC Importer GlnPQ and Determine the Rate of Transport.

Niosomes, an alternative for liposomal delivery.

Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells.

Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking.

(Membrane) Protein Production in Context.

How Important Is Protein Diffusion in Prokaryotes?

Coupling efficiency of secondary active transporters.

Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength.

Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress.

Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers.

Cell Fuelling and Metabolic Energy Conservation in Synthetic Cells.

A synthetic metabolic network for physicochemical homeostasis.

Regulation of Amino Acid Transport in Saccharomyces cerevisiae.

Flexible and Extended Linker Domains Support Efficient Targeting of Heh2 to the Inner Nuclear Membrane.

A Trifunctional Linker for Palmitoylation and Peptide and Protein Localization in Biological Membranes.

Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane.

Physiochemical Modeling of Vesicle Dynamics upon Osmotic Upshift.

Extracellular loops matter - subcellular location and function of the lysine transporter Lyp1 from Saccharomyces cerevisiae.

Periprotein lipidomes of provide a flexible environment for conformational changes of membrane proteins.

Enzymology of the pathway for ATP production by arginine breakdown.

Membrane Lipid Requirements of the Lysine Transporter Lyp1 from Saccharomyces cerevisiae.

Stereoselective Protection-Free Modification of 3-Keto-saccharides.

Kinetic Modelling of Transport Inhibition by Substrates in ABC Importers.

Structural and functional diversity calls for a new classification of ABC transporters.

Gating by ionic strength and safety check by cyclic-di-AMP in the ABC transporter OpuA.

Energy coupling of membrane transport and efficiency of sucrose dissimilation in yeast.

Growth Inhibition by Amino Acids in .

Physicochemical considerations for bottom-up synthetic biology.

Measurement of Protein Mobility in Reveals a Unique Tolerance to Osmotic Stress and Temperature Dependence of Diffusion.

In silico method for selecting residue pairs for single-molecule microscopy and spectroscopy.

Structural and biophysical characterization of the tandem substrate-binding domains of the ABC importer GlnPQ.

Glass-like characteristics of intracellular motion in human cells.

Chemogenetic Tags with Probe Exchange for Live-Cell Fluorescence Microscopy.

Stability of Ligand-induced Protein Conformation Influences Affinity in Maltose-binding Protein.

Heterodimer Formation of the Homodimeric ABC Transporter OpuA.

Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell.

Minimal Pathway for the Regeneration of Redox Cofactors.

Membrane thickness, lipid phase and sterol type are determining factors in the permeability of membranes to small solutes.

ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis.

Natamycin sequesters ergosterol and interferes with substrate transport by the lysine transporter Lyp1 from yeast.

Protein diffusion in cytoplasm scales with the mass of the complexes and is location dependent.

Determining small-molecule permeation through lipid membranes.

Super-resolving microscopy reveals the localizations and movement dynamics of stressosome proteins in Listeria monocytogenes.

Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective.

Physicochemical homeostasis in bacteria.

Simulation-based Reconstructed Diffusion unveils the effect of aging on protein diffusion in Escherichia coli.

Single-protein Diffusion in the Periplasm of Escherichia coli.

Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions.