Characteristics of Pain Changes in Rats with Nerve Injury Within 24 hours After One-Time Tuina Intervention

Our research scope belonged to the efficacy verification and the mechanism exploration of tuina therapy for neuropathic pain. We want to answer the effectiveness of tuina through animal experiments, providing scientific evidence for the analgesic efficacy of tuina in clinical practice. At present, RNA sequencing, calcium imaging, and the molecular biology technologies are used to promote further research in this field.

The analgesic efficacy of tuina, within 24 hours after intervention, is significantly different at different time points. This provides a scientific basis for selecting appropriate behavioral time points and sampling for further molecular mechanism exploration in the later study of the initiation mechanism of the Three-Method and Three-Acupoint"tuina analgesia. The research protocol further validates the immediate and sustained analgesic effects of Three-Method and Three-Acupoint"tuina technique and provide a more accurate time point for exploring the initiation mechanism of tuina analgesia in the later stage.

In subsequent research, we will continue to focus on exploring the initiation mechanism of analgesia by tuina in order to provide scientific evidence for clinical tuina treatment of neuropathic pain-related diseases.