1Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, 2Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, 3Institute of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, 4Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China
* These authors contributed equally
Summary
This article takes the phiC31 integrase-mediated transgenesis in Drosophila as an example and presents an optimized protocol for embryo microinjection, a crucial step for creating transgenic flies.
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