High-Throughput Screening to Obtain Crystal Hits for Protein CrystallographyGabrielle R. Budziszewski 1, M. Elizabeth Snell 1, Tiffany R. Wright 1, Miranda L. Lynch 1, Sarah E. J. Bowman 1,2
1National High-Throughput Crystallization Center, Hauptman-Woodward Medical Research Institute, 2Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, The State University of New York
This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.
