Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic ModificationLidiia Tynianskaia 1, Nesil Eşiyok 1, Wieland B. Huttner 2, Michael Heide 1,2
1German Primate Center, Leibniz Institute for Primate Research, 2Max Planck Institute of Molecular Cell Biology and Genetics
The electroporation of primate cerebral organoids provides a precise and efficient approach to introduce transient genetic modification(s) into different progenitor types and neurons in a model system close to primate (patho)physiological neocortex development. This allows the study of neurodevelopmental and evolutionary processes and can also be applied for disease modeling.
