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Key Laboratory of Anesthesiology (Shanghai Jiaotong University), Ministry of Education

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Preliminary Validation of Stereotaxic Injection Coordinates via Cryosectioning

Xiaoxin Zhou^*1,2,3, Wanbing Dai^*1,2, Jie Zhou^*1,2, Yizhe Zhang^1,2, Xiao Zhang^1,2, Sihan Chen^1,2, Xiaoyu Sun^1,2

¹Department of Anesthesiology, Renji Hospital, Shanghai Jiaotong University, School of Medicine, ²Key Laboratory of Anesthesiology (Shanghai Jiaotong University), Ministry of Education, ³Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiaotong University

The present protocol describes a practical strategy to expedite the verification step of stereotaxic injection coordinates before conducting viral tracing using dyes and frozen sections.