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Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry

2 ARTICLES PUBLISHED IN JoVE

Biology

In Vivo Leaf Inoculation: An Alternative Method to Assess the Disease Resistance of Hybrid Clones in Poplar Breeding of Stem Canker Disease

Yutian Suo^*1,2, Yinan Zhang^*1,2, Zheng Li^1,2, Mingzhuo Zhao³, Wanna Shen^1,2, Yuchen Fu^1,2, Donglin Wang^1,2, Long Pan^1,2, Xiaohua Su⁴, Jiaping Zhao^1,2

¹State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, ²Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry, ³Beijing Haidian Kaiwen Academy High School, ⁴State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry

We provide a step-by-step protocol for assessing poplar resistance to stem canker pathogens using an in vivo leaf inoculation method. This method is especially suitable for large-scale assessment of Cytospora chrysosperma and Botryosphaeria dothidea canker disease resistance in poplar breeding progeny in China.

Biology

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Min Li^*1,2, Donglin Wang^*1,2, Yuchen Fu^1,2, Mingzhuo Zhao³, Zheng Li^1,2, Wanna Shen^1,2, Long Pan^1,2, Xiaohua Su⁴, Jiaping Zhao^1,2

Here, we present a protocol to induce the production of adventitious roots (ARs) through the phloem- or epidermis-girdle fungal pathogen inoculation pathway, which is suitable for the study of root biology and the light response-related physiological processes in poplar.