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Kingsborough Community College

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Farshad Tamari¹, Joanna Tychowski², Laura Lorentzen³

¹Department of Biological Sciences, Kingsborough Community College, ²Institute for Cellular and Molecular Biology, University of Texas at Austin, ³New Jersey Center for Science, Technology and Mathematics, Kean University

We set out to determine tolerable concentrations of three fatty acids (oleic, linoleic and linolenic acids) and cholera toxin that did not significantly and adversely affect cell survival by solubilizing the fatty acids and the toxin and using them in cell survival assays.