ATAC-Seq Library Preparation of Murine Bone Marrow-Derived NeutrophilsJu Zhang *1,2, Shuai Tong *1,2, Siyuan Liang *1,2, Fangyuan Li 1,2, Can Zhang 1,2, Nan Ding 1,2, Yu Hao 1,2
1Biomedical Innovation Center, Beijing Shijitan Hospital, Capital Medical University, 2Beijing Key Laboratory for Therapeutic Cancer Vaccines
This manuscript outlines a protocol for preparing an ATAC-seq library of neutrophils from murine bone marrow, aiming to guarantee optimal neutrophil viability and high library quality. It offers step-by-step instructions on BMC preparation, immunomagnetic sorting, and library construction, serving as a valuable guide, especially for newcomers to studying neutrophils.
