Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle CryopreservationNina Desai 1, Megan Attard 1, Maribeth Spangler 1, Arsela Gishto 1, Alyssa Brown 1, Megan Wirtjes 1
1Cleveland Clinic Fertility Center
This protocol describes a novel three-dimensional (3-D) culture model using a tyramine-linked hyaluronan hydrogel to encapsulate and culture preantral follicles from the mouse ovary. We also detail two approaches to ovarian follicle cryopreservation by vitrification.
