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chinese academy of forestry

4 ARTICLES PUBLISHED IN JoVE

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Biology

Observation and Quantification of Mating Behavior in the Pinewood Nematode, Bursaphelenchus xylophilus
Najie Zhu *1, Liqun Bai *1, Stefan Schütz 2, Baojun Liu 3, Zhenyu Liu 3, Xingyao Zhang 4, Hongshi Yu 1, Jiafu Hu 1,5
1Department of Forest Protection, Zhejiang Agricultural & Forestry University, 2Institute of Forest Zoology and Forest Conservation, Georg-August University Göttingen, 3College of Plant Protection, Shandong Agricultural University, 4Institute of Forest Protection, Chinese Academy of Forestry, 5Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University

A protocol for investigating the mating behavior of the pinewood nematode, Bursaphelenchus xylophilus, is presented. Behavioral features of B. xylophilus are described in the mating process.

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Biology

Development of an Individual-Tree Basal Area Increment Model using a Linear Mixed-Effects Approach
Wenwen Wang 1, Yanfeng Bai 2, Chunqian Jiang 2, Jinghui Meng 1
1Research Center of Forest Management Engineering of National Forestry and Grassland Administration, Beijing Forestry University, 2Research Institute of Forestry, Chinese Academy of Forestry

Mixed-effects models are flexible and useful tools for analyzing data with a hierarchical stochastic structure in forestry and could also be used to significantly improve the performance of forest growth models. Here, a protocol is presented that synthesizes information relating to linear mixed-effects models.

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Biology

In Vivo Leaf Inoculation: An Alternative Method to Assess the Disease Resistance of Hybrid Clones in Poplar Breeding of Stem Canker Disease
Yutian Suo *1,2, Yinan Zhang *1,2, Zheng Li 1,2, Mingzhuo Zhao 3, Wanna Shen 1,2, Yuchen Fu 1,2, Donglin Wang 1,2, Long Pan 1,2, Xiaohua Su 4, Jiaping Zhao 1,2
1State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, 2Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry, 3Beijing Haidian Kaiwen Academy High School, 4State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry

We provide a step-by-step protocol for assessing poplar resistance to stem canker pathogens using an in vivo leaf inoculation method. This method is especially suitable for large-scale assessment of Cytospora chrysosperma and Botryosphaeria dothidea canker disease resistance in poplar breeding progeny in China.

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Biology

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes
Min Li *1,2, Donglin Wang *1,2, Yuchen Fu 1,2, Mingzhuo Zhao 3, Zheng Li 1,2, Wanna Shen 1,2, Long Pan 1,2, Xiaohua Su 4, Jiaping Zhao 1,2
1State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, 2Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry, 3Beijing Haidian Kaiwen Academy High School, 4State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry

Here, we present a protocol to induce the production of adventitious roots (ARs) through the phloem- or epidermis-girdle fungal pathogen inoculation pathway, which is suitable for the study of root biology and the light response-related physiological processes in poplar.

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