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Bejing University of Chinese Medicine

3 ARTICLES PUBLISHED IN JoVE

Biology

Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure

Kenneth K. Kwong^1,2, Lenuta Kloetzer^1,2,3,4, Kelvin K. Wong^5,6, Jia-Qian Ren^1,2, Braden Kuo^1,2,3,4, Yan Jiang⁷, Y. Iris Chen^1,2, Suk-Tak Chan^1,2,8, Geoffrey S. Young⁹, Stephen T.C. Wong^5,6

¹Department of Radiology, Massachusetts General Hospital, Harvard Medical School, ²Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, ³Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, ⁴Department of Medicine, Massachusetts General Hospital, Harvard Medical School, ⁵Center for biotechnology and Informatics, The Methodist Hospital Research Institute, ⁶Department of Radiology, The Methodist Hospital, Weill Cornell Medical College, ⁷Bejing University of Chinese Medicine, ⁸Department of Health Technology and Informatics, The Hong Kong Polytechnic University, ⁹Department of Radiology, Brigham and Women's Hospital, Harvard Medical School

Gua Sha, traditional Chinese therapeutic skin scraping, causes subcutaneous microvascular blood extravasation. We report a protocol of bioluminescence imaging of HO-1-luciferase transgenic mice to demonstrate that Gua Sha upregulates heme oxygenase-1 (HO-1) in multiple organs.

Developmental Biology

Using Immunofluorescence to Detect PM_2.5-induced DNA Damage in Zebrafish Embryo Hearts

Yujie Huang^*1, Yizhou Tao^*1, Chang Cai¹, Jin Chen¹, Cheng Ji¹, Stanley Aniagu², Yan Jiang¹, Tao Chen¹

¹School of Public Health, Soochow University, ²Toxicology, Risk Assessment and Research Division, Texas Commission on Environmental Quality

This protocol uses an immunofluorescence assay to detect PM_2.5-induced DNA damage in the dissected hearts of zebrafish embryos.

Neuroscience

Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

Zarmeen Mussa^1,2, Jessica Tome-Garcia^1,2, Yan Jiang³, Schahram Akbarian^2,4, Nadejda M. Tsankova^1,2

¹Department of Pathology, Icahn School of Medicine at Mount Sinai, ²Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, ³Institutes of Brian Science, Fudan University, ⁴Department of Psychiatry, Icahn School of Medicine at Mount Sinai

We have developed a method that enriches for and isolates human astrocyte populations from fresh-frozen tissue for use in downstream molecular analyses.