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The University of Texas Medical Branch

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
Guorui Xie 1, Melissa C. Whiteman 2, Jason A. Wicker 2, Alan D.T. Barrett 1,2,3, Tian Wang 1,2,3
1Department of Microbiology & Immunology, The University of Texas Medical Branch, 2Department of Pathology, The University of Texas Medical Branch, 3Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development, The University of Texas Medical Branch

Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in mice.

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Genetics

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Bahjat F. Marayati 1,2, James B. Pease 1, Ke Zhang 1,2
1Department of Biology, Wake Forest University, 2Center for Molecular Communication and Signaling, Wake Forest University

We present a simple suppressor screen protocol in fission yeast. This method is efficient, mutagen-free, and  selective  for  mutations that  often  occur at   a  single  genomic  locus.  The protocol is suitable for isolating suppressors that alleviate growth defects in liquid culture that are caused by a mutation or a drug.

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Immunology and Infection

A TNBS-Induced Rodent Model to Study the Pathogenic Role of Mechanical Stress in Crohn's Disease
Ramasatyaveni Geesala *1, You-Min Lin *1, Ke Zhang *1, Suimin Qiu 2, Xuan-Zheng Shi 1
1Department of Internal Medicine, the University of Texas Medical Branch, 2Department of Pathology, the University of Texas Medical Branch

The present protocol describes the development of a Crohn's-like colitis model in rodents. Transmural inflammation leads to stenosis at the TNBS instillation site, and mechanical enlargement is observed in the segment proximal to the stenosis. These changes allow studying mechanical stress in colitis.

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