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RWTH-Aachen University

2 ARTICLES PUBLISHED IN JoVE

Medicine

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

Kazuyuki Nagai^1,2, Shintaro Yagi², Shinji Uemoto², Rene H. Tolba¹

¹Institute for Laboratory Animal Science and Experimental Surgery, RWTH-Aachen University, ²Department of Hepato-Biliary-Pancreatic Surgery and Transplantation, Graduate School of Medicine, Kyoto University

Orthotopic liver transplantation in rats is an indispensable experimental model for biomedical research. Here we present our surgical procedures for orthotopic rat liver transplantation with hepatic arterial reconstruction using a 50% partial graft.

Immunology and Infection

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Felix Heymann^*1, Patricia M. Niemietz^*1, Julia Peusquens¹, Can Ergen¹, Marlene Kohlhepp¹, Jana C. Mossanen¹, Carlo Schneider¹, Michael Vogt², Rene H. Tolba³, Christian Trautwein¹, Christian Martin⁴, Frank Tacke¹

¹Department of Medicine III, RWTH University-Hospital Aachen, ²IZKF Aachen Core Facility "Two-Photon Imaging", RWTH University-Hospital Aachen, ³Institute for Laboratory Animal Science & Experimental Surgery, RWTH Aachen University, ⁴Institute for Pharmacology, RWTH University-Hospital Aachen

Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.