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3 ARTICLES PUBLISHED IN JoVE

Biology

¹UVP, LLC, ²Proteomic Center, Keck Graduate Institute of Applied Life Sciences

Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. This video provides protocols for protein separation, blotting proteins onto membranes, immunoprobing, and visualization using chromogenic or chemiluminescent substrates.

Biology

Staining Proteins in Gels

Sean Gallagher¹, Deb Chakavarti²

¹Keck Graduate Institute of Applied Life Sciences, UVP, LLC, ²Proteomic Center, Keck Graduate Institute of Applied Life Sciences

Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. Staining of proteins with Coomassie Blue, Silver Staining, SYPRO Orange, SYPRO Ruby are demonstrated in this video.

Cancer Research

Fluorescent Orthotopic Mouse Model of Pancreatic Cancer

Jonathan A. Moreno¹, Antonio Sanchez², Robert M. Hoffman^3,4, Saima Nur¹, Maria P. Lambros¹

¹College of Pharmacy, Western University of Health Sciences, ²UVP, LLC, ³Department of Surgery, School of Medicine, University of California San Diego, ⁴Anticancer Inc.

A procedure to implant green fluorescent protein-expressing pancreatic cancer cells (PANC-1 GFP) orthotopically into the pancreas of Balb-c Ola Hsd-Fox1nu mice to assess tumor progression and metastasis is presented here.