A Fluorescent Screening Assay for Identifying Modulators of GIRK ChannelsMaribel Vazquez 1, Charity A. Dunn 1, Kenneth B. Walsh 1
1Department of Pharmacology, Physiology & Neuroscience, University of South Carolina, School of Medicine
A real-time screening procedure for identifying drugs that interact with G protein-gated inward rectifier K+ (GIRK) channels is described. The assay utilizes membrane potential-sensitive fluorescent dyes to measure GIRK channel activity. This technique is adaptable for use on a number of cell lines.
