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Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

David R. Myers^*1,2,3,4, Yumiko Sakurai^*1,2,3,4, Reginald Tran^1,2,3,4, Byungwook Ahn^1,2,3,4, Elaissa Trybus Hardy^1,2,3,4, Robert Mannino^1,2,3,4, Ashley Kita^1,2,3,4, Michelle Tsai^1,2,3,4, Wilbur A. Lam^1,2,3,4

¹Department of Pediatrics, Emory University School of Medicine, ²Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ³Aflac Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta , ⁴Winship Cancer Institute of Emory University

A method to culture an endothelial cell monolayer throughout the entire inner 3D surface of a microfluidic device with microvascular-sized channels (<30 μm) is described. This in vitro microvasculature model enables the study of biophysical interactions between blood cells, endothelial cells, and soluble factors in hematologic diseases.