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Academy of Military Medical Sciences

4 ARTICLES PUBLISHED IN JoVE

JoVE Methods Collections: Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control

Charles E. Rupprecht¹, Wei-Cheng Hsu², Feng Ye³, Megan Golding⁴, Luca Zaeck⁵, Laurent Dacheux⁶, Noel Tordo⁷, Jodie A. Jarvis⁸, Erin Patrick⁹, Are Berentsen¹⁰

¹LYSSA LLC, ²Animal Health Research Institute, Council of Agriculture, Executive Yuan, ³Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, ⁴Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, ⁵Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, ⁶Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, ⁷Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, Institut Pasteur de Guinea, Conakry, ⁸Rabies Laboratory, Wadsworth Center, New York State Department of Health, ⁹Animal and Plant Health Inspection Service, Wildlife Services, Knoxville, TN, US Department of Agriculture, ¹⁰Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, US Department of Agriculture

JoVE Methods Collections: Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control

Developmental Biology

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Lin-Chen Li^*1,2, Xin-Xin Yu^*1,2, Yu-Wei Zhang^*1, Ye Feng^*1,3, Wei-Lin Qiu^*1,3, Cheng-Ran Xu¹

¹College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, ²Academy for Advanced Interdisciplinary Studies, Peking University, ³PKU-Tsinghua-NIBS Graduate Program, Peking University

We describe a method for the isolation of endocrine cells from embryonic, neonatal and postnatal pancreases followed by single-cell RNA sequencing. This method allows analyses of pancreatic endocrine lineage development, cell heterogeneity and transcriptomic dynamics.

Environment

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions

Zhongyi Wang^*1, Jiaming Li^*2, Lu’an Qian^*3, Linna Liu¹, Jun Qian¹, Bing Lu¹, Zhendong Guo¹

¹Academy of Military Medical Sciences, ²Chinese Academy of Agricultural Sciences, ³The Experimental High School Attached to Beijing Normal University

Understanding the biological composition of environmental particulate matter is important for the study of its significant impacts on human health and disease spread. Here, we used three types of bioaerosol sampling methods and a biological analysis of airborne microbes to better explore airborne microbial communities under different environmental conditions.

Genetics

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

Yuyang Wang^*1, Weidi Xu^*1,2, Huancheng Guo¹, Wenjie Gong¹, Biao He¹, Zhongzhong Tu¹, Changchun Tu^1,3, Ye Feng¹

¹Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, ²Jilin Agricultural University, ³Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

A pan-lyssavirus nested reverse transcription polymerase chain reaction has been developed to detect specifically all known lyssaviruses. Validation using rabies brain samples of different animal species showed that this method has a sensitivity and specificity equivalent to the gold standard fluorescent antibody test and could be used for routine rabies diagnosis.