Visualizing Antigen Specific CD4+ T Cells using MHC Class II TetramersEddie A. James 1, Rebecca LaFond 2, Ivana Durinovic-Bello 3, William Kwok 2
1Tetramer Core Laboratory, Benaroya Research Institute, 2Kwok Laboratory, Benaroya Research Institute, 3Nepom Laboratory, Benaroya Research Institute
This procedure demonstrates the purification and in vitro expansion of antigen specific CD4+ T cells from whole peripheral blood and their visualization using MHC class II tetramers. Tetramers permit the direct visualization of T cells with a single antigen specificity and defined MHC class II restriction.
