High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial CellsAlexander Braun 1, Nicole M. Caesar 1, Kyvan Dang 1, Kenneth A. Myers 1
1Department of Biological Sciences, University of the Sciences in Philadelphia
Protocols for Human Umbilical Vein Endothelial Cell (HUVEC) culture, transient transfection of fluorescently-labeled markers of microtubule growth, live-cell imaging and automated analysis of interphase microtubule growth dynamics are detailed.
