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U.S. Army Institute of Surgical Research

5 ARTICLES PUBLISHED IN JoVE

Biology

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

Whitney A. Greene¹, Alberto. Muñiz¹, Mark L. Plamper¹, Ramesh R. Kaini¹, Heuy-Ching Wang¹

¹Ocular Trauma, U.S. Army Institute of Surgical Research, JBSA Fort Sam Houston

The microRNA (miRNA) profiles of human induced-pluripotent stem (iPS) cells, retinal pigment epithelium (RPE) derived from human induced-pluripotent stem (iPS) cells (iPS-RPE), and fetal RPE, were compared.

Developmental Biology

Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies

Alberto Muñiz¹, Kaini R. Ramesh¹, Whitney A. Greene¹, Jae-Hyek Choi¹, Heuy-Ching Wang¹

¹Ocular Trauma, U.S. Army Institute of Surgical Research

The objective of this report is to describe the protocols to derive the retinal pigment epithelium (RPE) from induced pluripotent stem (iPS) cells using different sizes of embryoid bodies.

JoVE Journal

Physiology Lab Demonstration: Glomerular Filtration Rate in a Rat

Carmen Hinojosa-Laborde¹, Brian Jespersen², Robert Shade³

¹Tactical Combat Casualty Care Research, U.S. Army Institute of Surgical Research, ²Department of Pharmacology and Toxicology, Michigan State University, ³Southwest National Primate Research Center, Texas Biomedical Research Institute

The purpose of this protocol is to demonstrate the principles and techniques for measuring and calculating glomerular filtration rate, urine flow rate, and excretion of sodium and potassium in a rat. This demonstration can be used to provide students with an overall conceptual understanding of how to measure renal function.

Medicine

Integrated Compensatory Responses in a Human Model of Hemorrhage

Victor A. Convertino^2,3, Carmen Hinojosa-Laborde^1,2, Gary W. Muniz^1,2, Robert Carter, III^1,2

¹Tactical Combat Casualty Care Research, JBSA Fort Sam Houston, ²U.S. Army Institute of Surgical Research, JBSA Fort Sam Houston, ³U.S. Army Medical Research and Materiel Command, JBSA Fort Sam Houston

The purpose of this protocol is to demonstrate the techniques for measuring compensatory responses to reduced central blood volume using lower body negative pressure as a noninvasive experimental model of human hemorrhage which can be used to quantify the total integration of compensatory mechanisms to blood volume deficit in humans.

Developmental Biology

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Joni H. Ylostalo¹, Nikolay Bazhanov², Arezoo Mohammadipoor³, Thomas J. Bartosh⁴

¹Department of Biology, University of Mary Hardin-Baylor, ²Department of Pediatrics, University of Texas Medical Branch, ³Multi-Organ Support Technology Task Area, U.S. Army Institute of Surgical Research, ⁴Internal Medicine, Texas A&M University Health Science Center

The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.