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Institute of Hydrobiology, Chinese Academy of Sciences

3 ARTICLES PUBLISHED IN JoVE

Biology

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

Wei Hu^*1, Amin Tamadon^*1, Aaron J.W. Hsueh², Yi Feng¹

¹Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences; Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine, Fudan University, ²Program of Reproductive and Stem Cell Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford University

Here we present an adaptation of the passive CLARITY and 3D reconstruction method for visualization of the ovarian vasculature and follicular capillaries in intact mouse ovaries.

Immunology and Infection

Differentiation of Monocytes into Phenotypically Distinct Macrophages After Treatment with Human Cord Blood Stem Cell (CB-SC)-Derived Exosomes

Wei Hu^1,2, Xiang Song¹, Haibo Yu¹, Jingyu Sun², Yong Zhao¹

¹Center for Discovery and Innovation, Hackensack Meridian Health Center, ²Department of Chemistry and Chemistry Biology, Stevens Institute of Technology

Exosome application is an emerging tool for drug development and regenerative medicine. We establish an exosome isolation protocol with high purity to isolate exosomes from novel identified stem cells called CB-SC for mechanistic studies. We also coculture CB-SC-derived exosomes with human monocytes, leading to their differentiation into phenotypically distinct macrophages.

Developmental Biology

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

Wenyi Wang¹, Tao Kang¹, Lin Bai¹, Wei Hu², Yayoi Obata³, Jianzhen Li¹

¹College of Life Sciences, Northwest Normal University, ²State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, ³Department of Bioscience, Tokyo University of Agriculture

Here, we present a simple method for separating follicular cells and oocytes in zebrafish ovarian follicles, which will facilitate investigations of ovarian development in zebrafish.