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University of Sydney and Concord Hospital

1 ARTICLES PUBLISHED IN JoVE

Biology

A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy

Victoria C Cogger^*1,2,3, Jennifer N O'Reilly^*1,2, Alessandra Warren^1,2,3, David G Le Couteur^1,2,3

¹Centre for Education and Research on Ageing & ANZAC Research Institute, University of Sydney and Concord Hospital, ²Ageing and Alzheimers Institute, Concord Hospital, ³Charles Perkins Centre, University of Sydney

The fenestrated liver sinusoidal endothelial cell is a biologically important filter system that is highly influenced by various diseases, toxins, and physiological states. These changes significantly impact on liver function. We describe methods for the standardisation of the measurement of the size and number of fenestrations in these cells.