Abbvie
2 ARTICLES PUBLISHED IN JoVE
Biology
An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
Monica F. Poelchau 1, Xin Huang 1, Allison Goff 1, Julie Reynolds 2, Peter Armbruster 1
1Department of Biology, Georgetown University, 2Insect Physiology Lab, EEOB, The Ohio State University
RNA-Seq analyses are becoming increasingly important for identifying the molecular underpinnings of adaptive traits in non-model organisms. Here, a protocol to identify differentially expressed genes between diapause and non-diapause Aedes albopictus mosquitoes is described, from mosquito rearing, to RNA sequencing and bioinformatics analyses of RNA-Seq data.
Biology
High-throughput Measurement of Gut Transit Time Using Larval Zebrafish
Steven Cassar 1, Xin Huang 2, Todd Cole 3
1Preclinical Safety, AbbVie, 2Statistics, AbbVie, 3Integrated Science and Technology, AbbVie
The goal of this protocol is to measure the transit time of fluorescently labeled food through the gut of larval zebrafish in a high throughput fashion.
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