qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 GenotypingLeslie A. Mitchell 1, Nick A. Phillips 1, Andrea Lafont 2, James A. Martin 1, Rupal Cutting 2, Jef D. Boeke 1
1Department of Biochemistry and Molecular Pharmacology, Institute for Systems Genetics, 2Roche Life Science, USA
Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.
