Fabrication of a Multiplexed Artificial Cellular MicroEnvironment ArrayYasumasa Mashimo 1,2, Momoko Yoshioka 1, Yumie Tokunaga 1, Christopher Fockenberg 1, Shiho Terada 1, Yoshie Koyama 1, Teiko Shibata-Seki 2, Koki Yoshimoto 1, Risako Sakai 1, Hayase Hakariya 1, Li Liu 1, Toshihiro Akaike 3, Eiry Kobatake 2, Siew-Eng How 4, Motonari Uesugi 1,5, Yong Chen 1,6, Ken-ichiro Kamei 1
1Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 2Department of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 3Biomaterials Center for Regenerative Medical Engineering, Foundation for Advancement of International Science, 4Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 5Institute for Chemical Research, Kyoto University, 6Ecole Normale Supérieure
This article describes the detailed methodology to prepare a Multiplexed Artificial Cellular MicroEnvironment (MACME) array for high-throughput manipulation of physical and chemical cues mimicking in vivo cellular microenvironments and to identify the optimal cellular environment for human pluripotent stem cells (hPSCs) with single-cell profiling.
