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Ningbo University

12 ARTICLES PUBLISHED IN JoVE

Biology

Lens Transplantation in Zebrafish and its Application in the Analysis of Eye Mutants

Yan Zhang ^1,2, Kyle McCulloch², Jarema Malicki²

¹The Second Teaching Hospital of Jilin University, ²Department of Ophthalmology, Harvard Medical School

Lens development involves interactions with other tissues. Several zebrafish eye mutants are characterized by an abnormally small lens size. Here we demonstrate a lens transplantation experiment to determine whether this phenotype is due to intrinsic causes or defective interactions with tissues that surround the lens.

Neuroscience

Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture

Lin Jiajia^*1, Mak Shinghung^*2, Zheng Jiacheng¹, Wang Jialing¹, Xu Dilin¹, Hu Shengquan², Zhang Zaijun³, Wang Qinwen¹, Han Yifan^2,4, Cui Wei^1,2

¹Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, ²Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, ³Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, ⁴International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong

This protocol describes how to accurately measure neuronal viability using Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining in cultured cerebellar granule neurons, a primary neuronal culture used as an in vitro model in neuroscience and neuropharmacology research.

Behavior

Using Gold-standard Gait Analysis Methods to Assess Experience Effects on Lower-limb Mechanics During Moderate High-heeled Jogging and Running

Yan Zhang ^1,2,3, Meizi Wang¹, Jan Awrejcewicz³, Gusztáv Fekete⁴, Feng Ren¹, Yaodong Gu^1,2

¹Faculty of Sports Science, Ningbo University, ²Research Academy of Grand Health Interdisciplinary, Ningbo University, ³Department of Automation, Biomechanics and Mechatronics, The Lodz University of Technology, ⁴Savaria Institute of Technology, Eötvös Loránd University

This study investigated lower-limb kinematics and ground reaction force (GRF) during moderate high-heeled jogging and running. Subjects were divided into groups of experienced wearers and inexperienced wearers. A three-dimensional motion analysis system with a configured force platform captured lower-limb joint movements and GRF.

Medicine

Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

Xuehai Chen¹, Rong Yu², Ziqiang Xu³, Yan Zhang ³, Chengyang Liu⁴, Bicheng Chen^*1, Hao Jin^*3

¹Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, ²Reproductive Center, The First Affiliated Hospital of Wenzhou Medical University, ³Department of Transplantation, The First Affiliated Hospital of Wenzhou Medical University, ⁴Department of Surgery, Perelman School of Medicine at the University of Pennsylvania

Here, a protocol involving re-arterialized rat partial liver transplantation is presented. Specifically, 70% liver was resected in vivo by using an updated technique of vessel-oriented hepatectomy. The hepatic artery was reconstructed in an end-to-side manner. The cuff technique was modified to shorten the anastomosis time of the infrahepatic vena cava.

Biochemistry

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Huang Chunhui^1,2, Xu Dilin¹, Zhang Ke¹, Shentu Jieyi², Yan Sicheng¹, Wu Dapeng³, Wang Qinwen¹, Cui Wei^1,2

¹Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, ²Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, ³School of Materials Science and Chemical Engineering, Ningbo University

This protocol describes how to prepare Aβ oligomers from a synthetic peptide in vitro and to evaluate relative amounts of Aβ oligomer by a dot blotting analysis.

Behavior

Biomechanical Analysis Methods to Assess Professional Badminton Players' Lunge Performance

Ping Huang^1,2,3, Lin Fu¹, Yan Zhang ³, Gusztáv Fekete⁴, Feng Ren¹, Yaodong Gu^1,2

Here, we present a protocol to evaluate the differences in injury mechanisms between professional and amateur players when performing a badminton maximal right lunge movement by analyzing lower limb kinematics.

Neuroscience

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

Rui Wang¹, Zi-tong Huang³, Wen-kang Ren¹, Jiao Zhang¹, Yao Zhang¹, Bo Tan³, Ping Huang^1,2, Hong-ying Cao^1,2

¹School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, ²Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of Mathematical Engineering for Chinese Medicine, Guangzhou University of Chinese Medicine, ³School of Basic Medical Sciences, Guangzhou University of Chinese Medicine

This protocol attempts to establish a repeatable protocol for primary neurons and glia isolation from rat bladder for further cellular experiments.

Behavior

Comparative Analysis of Lower Limb Kinematics between the Initial and Terminal Phase of 5km Treadmill Running

Wenjing Quan¹, Meizi Wang², Gongju Liu², Gusztáv Fekete³, Julien S. Baker⁴, Feng Ren¹, Yaodong Gu¹

¹Faculty of Sports Science, Ningbo University, ²Faculty of Engineering, University of Pannonia Veszeprem, ³Savaria Institute of Technology, Eötvös Loránd University, ⁴Department of Sport, and Physical Education, Hong Kong Baptist University

This study investigated the biomechanical characteristics of the lower extremity kinematic variables between the initial and terminal phase of 5 km treadmill running. The lower-limb kinematic data of 10 runners were collected using a three-dimensional motion capture system on a treadmill at the initial phase (0.5 km) and the terminal phase (5 km), respectively.

JoVE Core

Lower-Limb Biomechanical Characteristics Associated with Unplanned Gait Termination Under Different Walking Speeds

Huiyu Zhou^1,2, Xuanzhen Cen¹, Yang Song³, Ukadike C. Ugbolue², Yaodong Gu¹

¹Faculty of Sports Science, Ningbo University, ²School of Health and Life Sciences, University of the West of Scotland, ³Faculty of Engineering, University of Szeged

This study compared the biomechanical characteristics of the lower extremity during unplanned gait termination under different walking speeds. The lower-limb kinematic and kinetic data from fifteen subjects with normal and fast walking speeds were collected using a motion analysis system and plantar pressure platform.

Behavior

Comparison of Kinetic Characteristics of Footwork during Stroke in Table Tennis: Cross-Step and Chasse Step

Huiyu Zhou^1,2, Yuqi He¹, Xiaoyi Yang¹, Feng Ren¹, Ukadike C. Ugbolue², Yaodong Gu¹

¹Faculty of Sports Science, Ningbo University, ²School of Health and Life Sciences, University of the West of Scotland

This study presents a protocol to investigate the ground reaction force characteristics between cross-step and chasse step during stroke in table tennis.

Medicine

Non-Intubated Video-Assisted Thoracoscopic Surgery

Gao Bin^*1,2, Luo Wenjun^*3, Gui Yu², Xu Zhipeng², Zhu Binbin², Yu Lina¹

¹Department of Anesthesia, Second Affiliated Hospital of Zhejiang University School of Medicine, ²Department of Anesthesia, The First Affiliated Hospital of Ningbo University, ³Health Science Center, Ningbo University

Here, we present a non-intubated protocol for performing video-assisted thoracoscopic surgery with preserved autonomic breathing.

Engineering

Embedded Bioprinting of Tissue-like Structures Using κ-Carrageenan Sub-Microgel Medium

Hua Zhang^*1,2,3, Tong Zhu^*1,2,4, Yang Luo^1,2, Rong Xu^1,2,4, Guanrong Li^1,2,4, Zeming Hu², Xu Cao^1,2,4, Jie Yao^3,4, Yige Chen^1,2, Yingchun Zhu⁴, Kerong Wu^3,4

¹Research Institute of Smart Medicine and Biological Engineering, Ningbo University, ²Health Science Center, Ningbo University, ³Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province, The First Affiliated Hospital of Ningbo University, ⁴The First Affiliated Hospital of Ningbo University

This study introduces a novel κ-carrageenan sub-microgel suspension bath, displaying remarkable reversible jamming-unjamming transition properties. These attributes contribute to the construction of biomimetic tissues and organs in embedded 3D bioprinting. The successful printing of heart/esophageal-like tissues with high resolution and cell growth demonstrates high-quality bioprinting and tissue engineering applications.