Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed ElutionGatikrushna Singh *1, Sarah M. Fritz *2, Arnaz Ranji 2, Deepali Singh 3, Kathleen Boris-Lawrie 1,2
1Department of Veterinary & Biomedical Sciences, University of Minnesota, 2Department of Veterinary Biosciences, Ohio State University, 3School of Biotechnology, Gautam Buddha University
This manuscript describes an approach to isolate select cognate RNPs formed in eukaryotic cells via a specific oligonucleotide-directed enrichment. We demonstrate the applicability of this approach by isolating a cognate RNP bound to the retroviral 5' untranslated region that is composed of DHX9/RNA helicase A.
