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National Jewish Health

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Charmion Cruickshank-Quinn¹, Kevin D. Quinn¹, Roger Powell¹, Yanhui Yang¹, Michael Armstrong¹, Spencer Mahaffey², Richard Reisdorph¹, Nichole Reisdorph¹

¹Department of Immunology, National Jewish Health, ²Department of Pharmacology, School of Medicine, University of Colorado Denver

The reliability of results in metabolomics experiments depends on the effectiveness and reproducibility of the sample preparation. Described is a rigorous and in-depth method that enables extraction of metabolites from biological fluids with the option of subsequently analyzing up to thousands of compounds, or just the compound classes of interest.

Immunology and Infection

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Mia J. Smith^1,2, Thomas A. Packard¹, Shannon K. O'Neill¹, Rochelle M. Hinman¹, Marynette Rihanek³, Peter A. Gottlieb³, John C. Cambier^1,4

¹Department of Immunology and Microbiology, University of Colorado School of Medicine, ²Department of Microbiology, Immunology, and Pathology, Colorado State University, ³Barbara Davis Center for Childhood Diabetes, University of Colorado School of Medicine, ⁴Department of Biomedical Research, National Jewish Health

A simple yet effective method that employs magnetic nanoparticles to detect and enrich antigen-reactive B cells for functional and phenotypic analysis is described.

Neuroscience

Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

Stacey M. Thomas¹, Joseph Bednarek², William J. Janssen¹, Patrick S. Hume¹

¹Division of Pulmonary, Sleep and Critical Care Medicine, Department of Medicine, National Jewish Health, ²Section of Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital Colorado

Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.