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Virginia-Maryland College of Veterinary Medicine

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice

Irving C. Allen¹

¹Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University

The host immune response to pathogen infection is a tightly regulated process. Utilizing a lipopolysaccharide lung exposure model in mice, it is possible to conduct high resolution evaluations of the complex mechanisms associated with disease pathogenesis.

Immunology and Infection

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

Daniel E. Rothschild¹, Tara Srinivasan², Linette A. Aponte-Santiago¹, Xiling Shen^2,3,4, Irving C. Allen¹

¹Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of Veterinary Medicine, Virginia Tech, ²Department of Biomedical Engineering, Cornell University, ³School of Electrical and Computer Engineering, Cornell University, ⁴Department of Biomedical Engineering, Duke University

Here, a protocol to harvest, maintain, and treat mouse small intestinal organoids with pathogen associated molecular patterns (PAMPs) and Listeria monocytogenes is described, as well as emphasis on gene expression and proper normalization techniques for protein.

Medicine

Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response

John A. Richey¹, Holly Sullivan-Toole², Marlene Strege¹, Corinne Carlton¹, Dylan McDaniel³, Matthew Komelski⁴, Amy Epperley⁵, Hongxiao Zhu⁶, Irving C. Allen^3,7

¹Department of Psychology, Virginia Tech, ²Graduate Program in Translational Biology, Medicine and Health, Virginia Tech, ³Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, ⁴Department of Human Development and Family Science, Virginia Tech, ⁵Wellness Center, Virginia Tech, ⁶Department of Statistics, Virginia Tech, ⁷Department of Basic Science Education, Virginia Tech Carilion School of Medicine

Minimal erythema dose (MED) testing is used to establish dosage schedules for ultraviolet radiation phototherapy. It can assess individual variation in inflammatory response but lacks methodology for achieving reproducible results. Here, we present a precision implementation of MED and demonstrate its ability to capture individual variation in inflammatory response.

Cancer Research

Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model

Margaret A. Nagai-Singer¹, Alissa Hendricks-Wenger^1,2,3, Rebecca M. Brock^1,2, Holly A. Morrison¹, Juselyn D. Tupik¹, Sheryl Coutermarsh-Ott¹, Irving C. Allen^1,2,4

¹Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, ²Graduate Program in Translational Biology, Medicine and Health, Virginia Polytechnic Institute and State University, ³Department of Biomedical Engineering and Mechanics, Virginia Polytechnic Institute and State University, ⁴Department of Basic Science Education, Virginia Tech Carilion School of Medicine, Virginia Polytechnic Institute and State University

We describe a more consistent and expeditious method to quantify lung metastasis in the 4T1 breast cancer model by using Fiji-ImageJ.