In Vitro Assessment of Cardiac Function Using Skinned CardiomyocytesPatrícia Gonçalves-Rodrigues *1, João Almeida-Coelho *1, Alexandre Gonçalves 1, Flávio Amorim 1, Adelino F. Leite-Moreira 1, Ger J.M. Stienen 2, Inês Falcão-Pires 1
1Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 2Department of Physiology, Kilimanjaro Christian Medical University College
This protocol aims to describe step-by-step the technique of extraction and assessment of cardiac function using skinned cardiomyocytes. This methodology allows measurement and acutemodulation of myofilament function using small frozen biopsies that can be collected from different cardiac locations, from mice to men.
