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Faculdade de Ciências da Universidade de Lisboa

3 ARTICLES PUBLISHED IN JoVE

Environment

BtM, a Low-cost Open-source Datalogger to Estimate the Water Content of Nonvascular Cryptogams

María Leo^*1, Angel Lareo^*2, Carlos Garcia-Saura², Joaquín Hortal^3,4, Nagore G. Medina⁵

¹Real Jardín Botánico (CSIC-RJB), ²Grupo de Neurocomputación Biológica, Dpto. de Ingeniería Informática, Escuela Politécnica Superior, Universidad Autónoma de Madrid, ³Department of Biogeography and Global Change, Museo Nacional de Ciencias Naturales (MNCN-CSIC), ⁴Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências da Universidade de Lisboa, ⁵Department of Botany, Faculty of Science, University of South Bohemia

We present a simple and cost-effective method to build an open-source datalogger that measures the conductance of nonvascular cryptogams together with the environmental temperature and humidity. We describe the hardware design of the datalogger and provide step-by-step assembly instructions, the list of required open-source logging software, the code to run the datalogger, and a calibration protocol.

Developmental Biology

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

André Dias¹, Gabriel G. Martins^1,2, Alexandre Lopes^1,3, Moisés Mallo¹

¹Instituto Gulbenkian de Ciência, ²Faculdade de Ciências da Universidade de Lisboa, ³NOVA School of Science and Technology

Here, we describe computational tools and methods that allow visualization and analysis of three and four-dimensional image data of mouse embryos in the context of axial elongation and segmentation, obtained by in toto optical projection tomography, and by live imaging and whole-mount immunofluorescence staining using multiphoton microscopy.

Biology

Infection of In Vivo and In Vitro Pines with the Pinewood Nematode Bursaphelenchus xylophilus and Isolation of Induced Volatiles

Jorge M. S. Faria^1,2, A. Cristina Figueiredo^3,4, Dora M. Teixeira⁵, Maria L. Inácio^1,2

¹INIAV, I.P., National Institute for Agrarian and Veterinary Research, I.P., ²GREEN-IT Bioresources for Sustainability, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB NOVA), ³Centre for Environmental and Marine Studies (CESAM Lisboa), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa, ⁴Centre for Ecology, Evolution and Environmental Changes (CE3C), Biotecnologia Vegetal (BV), DBV, Faculdade de Ciências da Universidade de Lisboa, ⁵Hercules Laboratory & Chemistry and Biochemistry Department of Science and Technology School, University of Évora

The protocol describes the in vivo and in vitro pinewood nematode infection of Pinus pinaster and their volatilome analysis through Gas Chromatography (GC) and GC coupled to Mass Spectrometry (GC-MS).