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University of Cambridge School of Clinical Medicine

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Assessment of Maternal Vascular Remodeling During Pregnancy in the Mouse Uterus

Jens Kieckbusch^1,2, Louise M. Gaynor^1,2, Francesco Colucci^1,2

¹Department of Obstetrics and Gynaecology, School of Clinical Medicine, University of Cambridge, ²Centre for Trophoblast Research, University of Cambridge

This protocol describes a technique to assess changes in the maternal vasculature during pregnancy in mice. Using stereological methods, remodeling of the decidual spiral arteries is assessed quantitatively and the results confirmed qualitatively using immunohistochemistry.

Immunology and Infection

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Delphine M. Depierreux^*1,2, Emily Seshadri^*1,2, Evgeniya V. Shmeleva^*1,2, Jens Kieckbusch^1,2, Delia A. Hawkes¹, Francesco Colucci^1,2

¹Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre, University of Cambridge School of Clinical Medicine, ²Centre for Trophoblast Research, University of Cambridge

This is a method to isolate uterine lymphoid cells from both pregnant and non-pregnant mice. This method can be used for multiple downstream applications such as FACS phenotyping, cell sorting, functional assays, RNA-seq, and proteomics. The protocol here demonstrates how to phenotype group 1 uterine innate lymphoid cells by flow cytometry.