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National Health Laboratory Service

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Rivak Punchoo^1,2, Esther Zhou¹, Sachin Bhoora¹

¹Department of Chemical Pathology, Faculty of Health Sciences, University of Pretoria, ²Tshwane Academic Division, National Health Laboratory Service

Apoptosis can be characterized by flow cytometric analysis of early and late apoptotic biomarkers. The cervical cancer cell line, SiHa, was analyzed for apoptosis biomarkers after treatment with Actinomycin D using a benchtop flow cytometer.

Immunology and Infection

Standard Membrane Feeding Assay for the Detection of Plasmodium falciparum Infection in Anopheles Mosquito Vectors

Erica Erlank^1,2, Nelius Venter^1,2, Lizette L. Koekemoer^1,2

¹Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, ²Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, National Health Laboratory Service

The standard membrane feeding assay (SMFA) is regarded as the gold standard for the assessment and identification of potential antimalarial compounds. This artificial feeding system is used to infect mosquitoes to further evaluate the effects of such compounds on the intensity and prevalence of the Plasmodium falciparum parasite.