Adult Pig Islet IsolationYing Lu *1,2, Zuhui Pu *3, Jiao Chen 2, Jing Deng 2, Ying Deng 2,4, Shufang Zhu 2, Chunliang Xu 5, Fuwen Yao 1,2, Zijing Wu 1,2, Yong Ni 1, Yongqiang Zhan 1, Jun Cheng 1, Naiyang Zhan 1, Wenlong Huang 6, Zhiming Cai 2, Rita Bottino 7, Lisha Mou 2
1Department of Hepatopancreatobiliary Surgery, Shenzhen Institute of Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Institute of Translational Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 3Imaging Department, Shenzhen Institute of Translational Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 4Faculty of Science, University of Waterloo, 5School of Life Science, The Chinese University of Hong Kong, 6General Surgery, The First Affiliated Hospital of Shantou University Medical College, 7Director Islet Programs, Imagine Pharma
This detailed protocol covers the methodological steps of adult pig islet isolation from the digestion phase via purification to the final functional assessment of the islets. This outline can be used as a guideline for adult pig islet isolation in research institutions.
