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Nottingham Trent University

2 ARTICLES PUBLISHED IN JoVE

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Biology

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
Kelly Yarnell 1, Rebecca S. Purcell 2, Susan L. Walker 2
1School of Animal Rural and Environmental Science, Nottingham Trent University, 2Chester Zoo, Upton-by-Chester

Adrenal activity can be assessed in the equine species by analysis of feces for corticosterone metabolites. The method offers a non-invasive option to assess long term patterns in both domestic and free ranging horses. This protocol describes the enzyme linked immunoassay involved and the associated biochemical validation.

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Bioengineering

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies
I-Ning Lee *1,2, Joseph Hosford *1, Shuai Wang 3, John A. Hunt 4, Judith M. Curran 2, William P. Heath 3, Lu Shin Wong 1
1Manchester Institute of Biotechnology & School of Chemistry, University of Manchester, 2School of Engineering, University of Liverpool, 3School of Electrical and Electronic Engineering, University of Manchester, 4School of Science and Technology, Nottingham Trent University

Here we present a protocol for wide-area scanning probe nanolithography enabled by the iterative alignment of probe arrays, as well as the utilization of lithographic patterns for cell-surface interaction studies.

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