Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow CytometryMeng-Fei Xu 1,2, Yu-Hao Yang 1,2, Ya-Lan Wei 3,4, Jing-Lian Zhang 1,2, Xuan Lin 1,2, Xiao-Yan Lin 1,2, Hui Chen 1,2, Zhen-Yu She 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University
This article reports an in vivo inhibition of CENP-E through abdominal surgery and testicular injection of GSK923295, a valuable model for male meiotic division. Using the immunofluorescence, flow cytometry and transmission electron microscopy assays, we show that CENP-E inhibition results in chromosome misalignment and genome instability in mouse spermatocytes.
