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University of California Los Angeles (UCLA)

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

¹Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, ²Research service, 151, Veterans Affairs Greater Los Angeles Healthcare System, ³Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics, University of California Los Angeles (UCLA), ⁴Division of Infectious Diseases, 111F, Veterans Affairs Greater Los Angeles Health Care System

An efficient method to assess surface-exposure of leptospiral proteins is described. The method is specifically designed to avoid disruption of the fragile outer membrane of leptospiral cells. This technique requires employment of several negative controls to assess the integrity of the outer membrane and specificity of antibody reaction.

Chemistry

Automation of a Positron-emission Tomography (PET) Radiotracer Synthesis Protocol for Clinical Production

Eric Schopf^*1, Christopher M. Waldmann^*2,3, Jeffrey Collins^2,4, Christopher Drake¹, Roger Slavik^2,3, R. Michael van Dam^2,4

¹SOFIE, ²Department of Molecular & Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles (UCLA), ³Ahmanson Translational Imaging Division, University of California, Los Angeles (UCLA), ⁴Crump Institute for Molecular Imaging, University of California, Los Angeles (UCLA)

Positron-emission tomography (PET) imaging sites that are involved in multiple early clinical research trials need robust and versatile radiotracer manufacturing capabilities. Using the radiotracer [¹⁸F]Clofarabine as an example, we illustrate how to automate the synthesis of a radiotracer using a flexible, cassette-based radiosynthesizer and validate the synthesis for clinical use.

Chemistry

Optimization of Radiochemical Reactions using Droplet Arrays

Alejandra Rios^1,2, Travis S. Holloway^2,3, Jia Wang^2,4, R. Michael van Dam^1,2,3,4

¹Physics and Biology in Medicine Interdepartmental Graduate Program, University of California Los Angeles (UCLA), ²Crump Institute of Molecular Imaging, UCLA, ³Department of Molecular & Medical Pharmacology, David Geffen School of Medicine, ⁴Department of Bioengineering, UCLA

This method describes the use of a novel high-throughput methodology, based on droplet chemical reactions, for the rapid and economical optimization of radiopharmaceuticals using nanomole amounts of reagents.