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Weill Medical College of Cornell University

2 ARTICLES PUBLISHED IN JoVE

Biology

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Elena Ivanova¹, Abduqodir H Toychiev¹, Christopher W Yee¹, Botir T Sagdullaev¹

¹Ophthalmology, Weill Medical College of Cornell University

The protocol described here is for structural assessment of a wholemount retinal preparation. This includes descriptions of tissue dissection, mounting onto a hydrophilized polytetrafluoroethylene (PTFE) membrane insert, bolus loading with fluorescent markers, and a comparison of fixation with carbodiimide and paraformaldehyde for immunohistochemical analysis of cellular and synaptic components.

Neuroscience

Whole-mount Imaging of Mouse Embryo Sensory Axon Projections

Kevin J. O’Donovan¹, Catherine O’Keeffe¹, Jian Zhong¹

¹Brain and Mind Research Institute, Burke Medical Research Institute, Weill Medical College of Cornell University

We present here an optimized protocol to genotype, stain and prepare fetal mice for the imaging of peripheral nociceptor axon projections in the whole animal, as an effective method to assess sensory axon growth phenotypes in developing genetically engineered mice.